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Objective To investigate the therapeutic effect of mycophenolate mofetil (MMF) on rats with experimental autoimmune encephalomyelitis (EAE).Methods The guinea pig spinal cord homogenate (GPSCH) and complete Freund's adjuvant were used as the antigen to induce the acute EAE model in Wistar rats which were verified according to clinical scoring.Totally 36 rats were randomly and equally divided into 3 groups,that is,normal control,EAE group and MMF treatment group.In 10 d after immunization,normal saline or MMF (30 mg?kg-1?d-1) were given intragastrically for 7 d to the corresponding groups.The rats were sacrificed on the 16th day and 30th day after immunization for pathological changes of the brain and spinal cord.Clinical manifestation was scored during the experiment.Results Compared with the EAE model group,MMF-treated rats had lower clinical scores and shorter disease process.Besides,the inflammatory infiltration,demyelination and axonal damage in the central nervous system were significantly improved in MMF treatment group.Conclusion MMF is an effective drug in treating EAE,and can relieve the clinical signs and ameliorate pathological lesions in the central nervous system of acute EAE rats.
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A survey was made in Chongqing Medical University on the cultural qualities of teachers of basic courses,their humanity and social knowledge,as well as the role these things have played in students' quality education.The analysis has showed that the cultural qualities of the teachers have been improved in the recent years,while there was still a gap from the requirements of cultivating students' cultural qualities.The survey suggests that it require great attention and constant efforts to improve the teachers' cultural qualities.
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Objective:To explore the correlation of ER expression to the activity of cell proliferation and clinicopathology in brain tumors(astrocytomas,ependynomas,and medulloblastomas).Methods:The expression of ER and proliferating cell nuclear antigen(PCNA) were determined by SP immunohistochemical method in 64 cases of brain tumors tissues.Correlation ER expression with chinicopathological characteristics was studied.Results:The positive rates of ER expression in astrocytomas,ependynomas,and medulloblastomas were 39.47%(15/38),35.71%(5/14) and 41.60%(5/12) respectively.Maliganat astrocytomas(42.8%) had a significantly higher ER expression rate compared with Benign astrocytomas(33.3%)(P
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Objective To explore the axonal damages in demyelination disease and its time window and morphological features of damaged axons. Methods Mouse model of multiple sclerosis (MS) was prepared by immunizing C57BL/6J mice with myelin oligodendrocyte glycoprotein peptides35-55 (MOG 35-55) to induce experimental autoimmune encephalomyelitis (EAE). Inflammation, demyelination and axonal damage at different time points of EAE including preonset, onset, peak and chronic were observed by common histology and specific stainings for the central nervous system. Results Demyelination and axonal damage occurred at the early stage of EAE, and was strongly related to inflammatory cell infiltration and demyelination of the central nervous system. Damaged axons showed the typical morphological features, including axon distortion, swelling, irregular diameter, axon transection with the ends ovoid, or bulb, and even axon disappearance. Conclusion Axonal damage is an early and common event in demyelination disease, and many factors might contribute to axonal damage.